Fig. 6: Requirement of DdSnc1 for PMS formation.
a Box plot showing the diameter of CRs formed by the WT, ΔDdSnc1, and DdSnc1C (n = 8 CRs for WT and ΔDdSnc1, and n = 6 CRs for DdSnc1C). The diameter was measured at d1, d2, and d3 noted in the schematic diagram. Two-tailed t-test. ns not significant (P = 0.06, 0.16 for ΔDdSnc1, DdSnc1C respectively). The middle line in the boxplot displays the median, edges represent the upper and lower quartiles, and whiskers indicate minimum and maximum values. Source data are provided as a Source Data file. Data shown are representatives from experiments performed in triplicate. b The ring cells (R1, R2, and R3) and stalk cells (S1 and S2) of CRs formed by WT, ΔDdSnc1, and DdSnc1C were stained with CFW. Scale bars = 6 µm. c Scanning electron micrographs of CRs before (upper panel) and after (lower panel) heat stimulation. F1 and F2 denote the first and second fusion points, respectively. Scale bars = 10 µm. d Transmission electron micrographs of the WT, ΔDdSnc1, and DdSnc1C ring cells. Black arrows indicate the thickened inner wall of ring cells. Magnified views of the areas denoted using the white dotted box are shown on the bottom or right side. Scale bars = 0.5 µm. These images represent what was observed among at least 5 CRs.
