Fig. 5: Overall structure of the Mm GS-GlnK1 complex.
a Ribbon diagram of the Mm GS-GlnK1 complex with the GS dodecamer colored green and the two GlnK trimers colored red. Shown are two views of the complex related by a rotation of 90°. b Ribbon diagram of the GS-GlnK1 interaction zoomed out to show the electrostatic contacts between GlnK1 residues Arg21 and Lys25 and GS residue Glu19. The T-loop contacts are included to indicate their relative to the GlnK1 trimer body. c Close-up of the GlnK1 T-loop binding site in GS. T-loop residues are shown as sticks and colored red. The two interacting GS subunits are colored green and labeled. Also shown is the 2mFo-DFc map contoured at 0.7 σ around the GS interacting GlnK1 T-loop residues. To avoid bias, the map shown is that calculated before addition of the GlnK1 residues including the T-loop.
