Fig. 1: Simultaneous detection of numerous differentiated cells and specialized neoblast classes by MERFISH.

a MERFISH labeling of major tissue classes in a thin sagittal section. Cartoon represents spatial location of depicted region. Scale bar, 250 µm; inset region (right), 100 µm. b Identification of specialized neoblasts by MERFISH. Colored circles represent identified specialized neoblasts. Scale bar, 100 µm. Inset depicts a representative detected specialized neoblast. Images represent fate signatures comprised of multiple gene targets described in Supplementary Table 2. Scale bar, 10 µm. Representative images from a single tissue section are shown in a and b from n = 3 animals. c Distances of identified specialized neoblasts to nearest mature tissues. Each condition contains measurements from specialized neoblasts of a given class within the region depicted in b, except for protonephridial specialized neoblasts which were measured in nearby anatomical regions. The upper and lower hinges correspond to 25th and 75th percentiles, whiskers represent the smallest and largest values, and the line corresponds to the median. n = 17 (Epidermal SpNB), 27 (Intestinal SpNB), 20 (Neural SpNB), 15 (BW Muscle SpNB), 11 (Protonephridial SpNB). SpNB, specialized neoblasts. Source data are provided as a Source Data file.