Fig. 5: Fate specification is intermingled in regeneration.

a Different fates can be specified in neighboring neoblasts at wound sites in regeneration. Pairs identified at anterior-facing wounds 72 hours post amputation. Scale bars, 10 µm. Representative images from n = 10 animals. Additional examples available in Supplementary Fig. 27. Arrows depict identified specialized neoblasts. Genes for tissue-specific FSTF pools: epidermis (soxP-3), intestine (hnf-4, gata4/5/6-1), muscle (myoD, snail), CNS (pax6A), eye (ovo); see also Supplementary Table 3. b Intermingled specialized neoblasts of distinct fate can be observed in an anterior-facing blastema 72hpa. Scale bar, 50 µm. c Intermingled specialized neoblasts can be observed in a posterior-facing blastema 96hpa. Scale bar, 50 µm. Representative images from n = 4 animals in b and c. Additional examples available in Supplementary Fig. 28. d Neighborhood analysis of intestinal and muscle-specialized neoblasts at anterior-facing wound sites 72hpa. N values provided in Supplementary Table 4. e Neighborhood analysis of eye-specialized neoblasts at anterior-facing wound sites 72hpa. N values provided in Supplementary Table 4. f Specialized neoblasts identified in in a transverse section of an animal 5 days post sagittal amputation. Dotted line represents boundary between old pre-existing tissue (left) and regenerating tissue (right). Scale bar, 100 µm. Representative image from n = 3 animals. g Distance measurements of ventral neural specialized neoblasts to ventral nerve cords or parenchymal cell types in old and regenerating tissue. N = 36 in old (left) and 31 in regenerating (right) tissues. Two-sided Welch’s t-test, ****p < 0.0001. The central line represents the mean and error bars represent standard deviation. Colored circles in b, c, and f depict identified specialized neoblasts. Int intestinal, Epi epidermal, Mus muscle, VNC ventral nerve cord, Par parenchymal cell types. Source data are provided as a Source Data file.