Fig. 2: Metagenomic traits along a pH gradient. | Nature Communications

Fig. 2: Metagenomic traits along a pH gradient.

From: Bacterial genome size and gene functional diversity negatively correlate with taxonomic diversity along a pH gradient

Fig. 2

a Bacterial average genome size (AGS) decreased as pH changed from acid to neutral.Bacterial AGS are detected by analyzing shotgun metagenome using MicrobeCensus pipeline. b Bacterial average 16S rRNA gene copy number (ACN) not significantly associated with soil pH. Bacterial ACN are detected by analyzing shotgun metagenome using the method from ref. 15. c Bacterial GC content (GC%) increased as pH changed from acid to neutral. Bacterial GC% are detected by analyzing shotgun metagenome using Quast software. d Bacterial estimated growth rate was unaffected by soil pH. Bacterial growth rate (minimal doubling time) was detected by analyzing shotgun metagenome using the gRodon pipeline. Linear regression model with two-sided test was used for the statistical analysis, and adjusted R-squared was used. n = 36 samples. The grey area around the smooth line indicates the 95% confidence interval. Source data are provided as a Source Data file.

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