Fig. 2: FADS2 activity contributes to the induction of trained immunity.
From: Fatty acid desaturation and lipoxygenase pathways support trained immunity
a Monocyte FADS1, FADS2, SCD gene expression 4 h and 24 h after BCG exposure (n = 8, except for FADS2 at 4 h which is n = 10 biologic replicates). b Schematic representation of the in vitro model used for the pharmacological inhibition of the induction of BCG-trained immunity. c Production of TNFα and IL-6 by BCG-trained macrophages incubated with (5 µM, 15 µM) SC-26196 for the first 24 h of culture and restimulated at day 6 with LPS for 24 h. (n = 6 biologic replicates pooled from 2 independent experiments). d Production of TNFα and IL-6 by BCG-trained macrophages incubated with (5 µM) GSK2033 for the first 24 h and restimulated with LPS at day 6 of culture for further 24 h. (n = 6 biologic replicates pooled from 2 independent experiments). Mean + SD. c, d two-way ANOVA, Sidak’s multiple comparisons test. (Ctrl: control; BCG: Bacillus Calmette-Guérin; LPS: lipopolysaccharide; FADS: fatty acid desaturase SCD: stearoyl-CoA desaturase).
