Fig. 5: Ion mobility separation of TnC monomer and cTn dimer Ca(II)-bound proteoforms by native TIMS-MS. | Nature Communications

Fig. 5: Ion mobility separation of TnC monomer and cTn dimer Ca(II)-bound proteoforms by native TIMS-MS.

From: Structure and dynamics of endogenous cardiac troponin complex in human heart tissue captured by native nanoproteomics

Fig. 5

a TnC monomer proteoforms (z = 7+) from TIMS-MS analysis. (Inset) Associated ion mobility heat map showing differences in ion mobility and intensity for each different Ca(II)-bound proteoform. b Collision cross section (CCS) of the TnC proteoforms obtained under nitrogen (N2) drift gas. c cTn dimer proteoforms (z = 13+) from TIMS-MS. (Inset) Associated ion mobility heat map showing differences in ion mobility and intensity for each different Ca(II)-bound proteoform. d CCS of the observed cTn dimer proteoforms obtained under N2 drift gas. e Illustrations of TnC monomer and (f) cTn dimer conformational changes upon Ca(II)-binding.

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