Fig. 3: Transcription of proU in the absence of StpA. | Nature Communications

Fig. 3: Transcription of proU in the absence of StpA.

From: The environmentally-regulated interplay between local three-dimensional chromatin organisation and transcription of proVWX in E. coli

Fig. 3

The RT-qPCR profile of the proVWX operon and its flanking regions in NT331 ΔstpA a during exponential growth at 0.08 M NaCl and b the fold-change in the transcript levels of the amplicons compared to NT331. c The RT-qPCR profile of the proVWX operon and its flanking regions in NT331 ΔstpA upon a hyperosmotic shock from 0.08 M to 0.3 M NaCl, and d the fold-change in transcript levels of the amplicons in comparison to exponential growth at 0.08 M NaCl. e The RT-qPCR profile of the proVWX operon and its flanking regions in NT331 ΔstpA during exponential growth at 0.3 M NaCl, and the fold-change in transcript levels of the amplicons with respect to f exponential growth at 0.08 M NaCl, and g a hyperosmotic shock. h The fold difference in transcript level of amplicon proU1 between NT331 ΔstpA and NT331. The fold change in transcript levels of the nrdF and ygaY amplicons between NT331 ΔstpA and NT331 i during exponential growth at 0.08 M NaCl, j exponential growth at 0.3 M NaCl, and k following a hyperosmotic shock. Y-axes: All bar graphs and data points with error bars show relative transcript levels in arbitrary units and are plotted on the left y-axis. Plots without error bars show fold-change in transcript level and correspond to the right y-axis. Internal control: rpoD. See also Supplementary Fig. 7. Data (3a–k) are presented as mean values +/- standard deviation. Dot plots (3a, 3c, 3e): n = 3 technical replicates of a biologically independent culture. Bar graphs (3a–k): n = 4 biologically independent cultures. Source data are provided as a Source Data file.

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