Table 1 Cryo-EM data collection, refinement and validation statistics of bacterial fibrils
| Â | Flagella (EMD-36427, PDB 8JMV) | Vpr (EMD-36428, PDB 8JMW) |
|---|---|---|
Data collection and processing | ||
 Magnification | ×130,000 | ×130,000 |
 Voltage (kV) | 300 | 300 |
 Electron exposure (e–/Å2) | 40 | 40 |
 Defocus range (μm) | 0.6–4.6 | 0.6–4.6 |
 Pixel size (Å) | 1.05 | 1.05 |
 Symmetry imposed | C1 | C2 |
 Helical rise (Å) | 4.8 | 20.5 |
 Helical twist (°) | 65.4 | −68.8 |
 Initial particle images (no.) | 318,365 | 318,365 |
 Final particle images (no.) | 153,285 | 76,671 |
 Map resolution (Å) | 2.9 | 2.9 |
  FSC threshold | 0.143 | 0.143 |
 Map resolution range (Å) | 200-2.9 | 200-2.9 |
Refinement | ||
 Initial model used (PDB code) | 5WJT | De novo |
 Model resolution (Å) | 3.1 | 3.1 |
  FSC threshold | 0.5 | 0.5 |
 Model resolution range (Å) | 200-3.1 | 200-3.1 |
 Map sharpening B factor (Å2) | 69 | 34 |
 Model composition | ||
  Non-hydrogen atoms | 800,025 | 69,552 |
  Protein residues | 10,692 | 9,306 |
  Ligands | 0 | 0 |
 B factors (Å2) | ||
  Protein | 73.75 | 89.16 |
  Ligand | – | – |
 R.m.s. deviations | ||
  Bond lengths (Å) | 0.007 | 0.007 |
  Bond angles (°) | 1.034 | 0.640 |
 Validation | ||
  MolProbity score | 1.87 | 1.87 |
  Clashscore | 12.85 | 9.12 |
  Poor rotamers (%) | 0 | 0 |
 Ramachandran plot | ||
  Favored (%) | 96.25 | 94.35 |
  Allowed (%) | 3.75 | 5.65 |
  Disallowed (%) | 0 | 0 |
