Fig. 3: Acbp6 regulates acetyl-CoA metabolism and protein acetylation to adjust midgut proliferative homeostasis during nutrition adaptation. | Nature Communications

Fig. 3: Acbp6 regulates acetyl-CoA metabolism and protein acetylation to adjust midgut proliferative homeostasis during nutrition adaptation.

From: A distinct Acyl-CoA binding protein (ACBP6) shapes tissue plasticity during nutrient adaptation in Drosophila

Fig. 3: Acbp6 regulates acetyl-CoA metabolism and protein acetylation to adjust midgut proliferative homeostasis during nutrition adaptation.

a Diagram of select genes and metabolites involved in acyl-CoA metabolism, acetyl-CoA metabolism, and acetylation. b–d Changes in various metabolite levels after whole-body upregulation of Acbp6 function. Genotypes; w1118; Daughterless (Da)Gal4/+ (controls, Ctrl.) and w1118; UAS-Acbp6/+; DaGal4/+. (5 whole flies per sample, n = 5 independent samples.) b Quantification of ATP (adenosine triphosphate) levels. c Quantification of citrate synthesis activity. d Quantification of acetyl-CoA levels. e Acetylated (Ac)-lysine immunostaining of dissected posterior midguts upon enterocyte-specific depletion of Acbp6 during nutrient adaptation, stained with anti-Ac-Lysine (green) and DAPI (nuclei, blue). Genotypes; w1118; NP1Gal4/+ (controls, Ctrl.) and w1118; NP1Gal4/UAS-Acbp6RNAi. f–h Genetic requirement of ACLY and CPT1 in Acbp6-dependent control of proliferative homeostasis during nutrient adaptation. f Quantification of phospho-Histone (H3) positive cells (per whole dissected midgut; from left to right, bars represent n = 15, 15, 14, 17, 15, 14, 15, 16, 12, 15, 17 and 16 independent samples), and immunostaining to detect g Delta positive cells in dissected posterior midguts (anti-Delta [red] and DAPI [nuclei; blue]), and h acetylated-lysine in dissected posterior midguts (stained with anti-Ac-Lysine (green) and DAPI (nuclei, blue) upon enterocyte-specific upregulation of Acbp6 or upregulation of Acbp6 and concurrent depletion of ACLY or CPT1 during nutrient adaptation (n = 3 independent experiments). Genotypes; w1118; NP1Gal4/+ (controls, Ctrl.), and w1118; NP1Gal4/UAS-Acbp6, and w1118; NP1Gal4, UAS-Acbp6/UAS-CPT1RNAi, and w1118; NP1Gal4, UAS-Acbp6/UAS-ACLYRNAi. Bars represent mean ± SEM (unpaired 2-tailed Student’s t-test). The exact p values are provided in figure. Source data are provided as a Source Data file. Fasting represents 2 days of nutrient deprivation, and Refeeding represents 2 days of refeeding. Scale bars, 10 μm.

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