Fig. 5: Analysis of Ko424 as ammonia donor for growth of Chlorella sorokiniana.

a–c Growth promotion of the eukaryotic algae Chlorella sorokiniana by Ko424 and Ko in N-free L agar at constant 30 °C, constant 23 °C or with 12 h temperature shifts between 30 °C and 23 °C (30 °C−23 °C), respectively. Strains were streaked in a fan-like arrangement on the plates with C. sorokiniana alternating between Ko424 and Ko as indicated. Plates were incubated in transparent anaerobic tanks and grown under light illumination for 7 days. d Chlorophyll a content of C. sorokiniana adjacent to Ko424 at constant 30 °C, constant 23 °C or with 12 hr temperature shifts between 30 °C and 23 °C (30 °C−23 °C), respectively. The means and SDs were calculated. n = 4 biologically independent samples. Statistical significance is indicated as **P ≤ 0.01 analyzed using one-way ANOVA with Student’s t-test. P values from left to right: 0.000000094 (30 °C vs 23 °C), 0.00000000029 (30 °C vs 30–23 °C), and 0.00107 (23 °C vs 30–23 °C). e–g Growth of the eukaryotic algae Chlorella sorokiniana, Ko424, and Ko in L agar supplemented with 3 mM ammonium at constant 30 °C, constant 23 °C or with 12 hr temperature shifts between 30 °C and 23 °C (30 °C−23 °C), respectively. Plates were incubated in transparent anaerobic tanks and grown under light illumination for 7 days. h Chlorophyll a content of C. sorokiniana adjacent to Ko424 at constant 30 °C, constant 23 °C or with 12 hr temperature shifts between 30 °C and 23 °C (30 °C−23 °C), respectively. The means and SDs were calculated. n = 4 biologically independent samples. Statistical significance is indicated as **P ≤ 0.01 analyzed using one-way ANOVA with Student’s t-test. NS, non-significant. P values from left to right 0.0087 (30 °C vs 23 °C) and 0.011 (30 °C vs 30–23 °C). Source data are provided as a Source Data file.