Table 1 Influence of temperature on growth parameters, nitrogenase activity and ammonia excretion by Ko and Ko424

From: Diurnal switches in diazotrophic lifestyle increase nitrogen contribution to cereals

 

Doubling time (min)

Glutamine (mM)

Glutamate (mM)

Nitrogenase activity (nmol min−1 mg−1)

Maximum NH4+ excretion (mM)

Ko

Ko424

Ko

Ko424

Ko

Ko424

Ko

Ko424

Ko

Ko424

33 °C

281 ± 25

438 ± 19

7.9 ± 0.1

1.6 ± 0.1

54 ± 1

34 ± 1

10.7 ± 1.1

36.5 ± 1.3

N.D.

N.D.

30 °C

255 ± 10

477 ± 19

8.8 ± 0.7

1.5 ± 0.2

58 ± 2

35 ± 1

23.3 ± 0.9

35.1 ± 1.4

N.D.

0.21 ± 0.03

27 °C

366 ± 18

602 ± 24

6.3 ± 0.3

1.3 ± 0.2

45 ± 1

31 ± 1

19.2 ± 0.6

31.8 ± 1.1

N.D.

1.77 ± 0.05

25 °C

487 ± 16

747 ± 26

5.2 ± 0.2

1.0 ± 0.1

38 ± 1

28 ± 1

14.6 ± 0.7

28.4 ± 0.9

N.D.

2.32 ± 0.11

23 °C

614 ± 27

908 ± 29

4.4 ± 0.2

0.7 ± 0.2

33 ± 1

26 ± 1

9.7 ± 1.3

24.8 ± 1.7

N.D.

2.98 ± 0.08

20 °C

783 ± 35

1375 ± 47

3.6 ± 0.1

0.5 ± 0.1

27 ± 1

23 ± 1

4.4 ± 0.6

20.9 ± 1.2

N.D.

3.32 ± 0.15

  1. Note that the ammonia excretion values do not correspond to the final ammonium concentrations in Supplementary Fig. 2C and D, since maximum ammonium excretion is observed after a much longer incubation period. Ko, inoculation with the wild-type strain of K. oxytoca; Ko424, inoculation with the GS-P95L mutant strain of K. oxytoca. The means and SDs were calculated based on at least three biological replicates. N.D. indicates not detectable. Source data are provided as a Source Data file.
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