Table 1 Kinetic parameters of NanoLuc luciferase mutants
From: Illuminating the mechanism and allosteric behavior of NanoLuc luciferase
Enzyme variant | Km/μM | kcat/s−1 | Kp/μM | kcat/Km/s−1 μM−1 |
|---|---|---|---|---|
CTZ-luminescence | ||||
NanoLuc | 0.57 ± 0.02 | 2.48 ± 0.05 | 0.256 ± 0.005 | 4.4 ± 0.2 |
NanoLuc-Y94A | 3.74 ± 0.09 | 0.225 ± 0.004 | 0.79 ± 0.04 | 0.060 ± 0.001 |
NanoLuc-D9R/K89R | 0.46 ± 0.01 | 16.8 ± 0.6 | 0.163 ± 0.006 | 37 ± 1 |
NanoLuc-D9R/H57A/K89R | 0.77 ± 0.07 | 40 ± 4 | 0.23 ± 0.02 | 70 ± 7 |
FMZ-luminescence | ||||
NanoLuc | 0.123 ± 0.004 | 7.88 ± 0.03 | 0.56 ± 0.01 | 64 ± 2 |
NanoLuc-Y94A | 1.29 ± 0.02 | 0.519 ± 0.007 | 0.85 ± 0.02 | 0.402 ± 0.006 |
NanoLuc-D9R/K89R | 0.157 ± 0.009 | 5.2 ± 0.1 | 0.39 ± 0.03 | 33 ± 2 |
NanoLuc-D9R/H57A/K89R | 0.098 ± 0.005 | 2.74 ± 0.03 | 0.34 ± 0.02 | 28 ± 1 |
