Fig. 1: Slc35d3 expression was lower in the fat tissues of obese mice.
From: SLC35D3 promotes white adipose tissue browning to ameliorate obesity by NOTCH signaling
a RT-qPCR analysis of Slc35d3 levels in EpiWAT, BAT, IngWAT, AsWAT, heart, spleen, liver, kidney, and brain from WT (n = 6) mice. b Representative western blot of Slc35d3 and β-Actin protein in EpiWAT, BAT, IngWAT, AsWAT, heart, spleen, liver, kidney, and brain from WT (n = 3) mice. The summary of the quantifications of three independent experiments is shown in the right panel. c Schematic diagram of ob/ob mouse treatment. d RT-qPCR analysis of Slc35d3 levels in IngWAT, EpiWAT, AsWAT and BAT from ob/ob (n = 6) and WT (n = 6) mice. e Representative western blot of Slc35d3 and β-Actin protein in IngWAT, EpiWAT, and BAT from ob/ob (n = 3) and WT mice (n = 3). Right panel, the summary of quantifications of three independent experiments. f DIO mice were fed an NCD from 4 to 6 weeks of age and a HFD from 6 to 20 weeks of age. g RT-qPCR analysis of Slc35d3 levels in IngWAT, EpiWAT, AsWAT, and BAT from DIO (n = 6) and WT (n = 6) mice. h Representative western blot of Slc35d3 and β-Actin protein in IngWAT, EpiWAT, and BAT from DIO (n = 3) and WT (n = 3) mice. Right panel, summary of quantifications of three independent experiments. Values are the fold induction of gene expression levels normalized to the housekeeping gene β-Actin (a, b, d, e, g, h), and data are expressed as the mean ± SEM. Statistical significance was assessed by unpaired two-sided Student’s t test (d, e, g, h). The exact P values are shown in the figure. EpiWAT epididymal white adipose tissue, BAT brown adipose tissue, IngWAT inguinal white adipose tissue, AsWAT anterior-subcutaneous white adipose tissue, NCD normal chow diet, HFD high-fat diet. Source data are provided as a Source Data file.
