Fig. 1: Cryo-EM of N-RNA from HMPV. | Nature Communications

Fig. 1: Cryo-EM of N-RNA from HMPV.

From: Structure of the N-RNA/P interface indicates mode of L/P recruitment to the nucleocapsid of human metapneumovirus

Fig. 1

A Size-exclusion chromatogram of recombinant HMPV N-RNA purified over a Superose 6 column. A protein standard is plotted as dotted line for comparison. B Representative 2D-class averages from cryo-EM. Particles were found predominantly as 10-mer oligomers. However, 11-mers (black arrow), 12-mers (white arrow), and spiral particles (red arrow) were also observed. C–E Cryo-EM maps of reconstructions of the indicated N-RNA oligomers. The bound RNA, and the C-terminal and N-terminal domains of N are labeled (CTD and NTD, respectively). The RNA is colored in yellow, the CTD in light blue, and the NTD in dark blue. F Tilted view and G side view of rigid body fitted N-RNA protomer models of the spiral assembly. N is shown in a cartoon representation, RNA is displayed as yellow spheres. The N protomers are colored sequentially from white to blue. Source data are provided as a Source Data file.

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