Fig. 2: Abnormal myelin sheath integrity and fewer oligodendrocytes in an Anks1b haploinsufficiency mouse model.
From: ANKS1B encoded AIDA-1 regulates social behaviors by controlling oligodendrocyte function
a Nissl staining of Anks1b Nestin-Het (n = 6) and WT (n = 8) mice shows decreased area of the corpus callosum. Images are representative of 18 sections from each mouse with acute slices from both genotypes placed side by side; scale bar = 1 mm; (p = 0.007463). b LFB (Luxol fast blue) staining for myelin sheath components reveals decreased density in corpus callosum of male and female Anks1b Nestin-Het mice compared to controls. Images are representative of 5 sections from each mouse; scale bar = 1 mm; (*p = 0.006713, ***p = 0.000357). c Transmission Electron Microscopy (TEM) images of callosal cross-sections from WT and Nestin-Het mice showing myelinated axons; scale bar = 1 µm. d Quantitation of g-ratio (the ratio of the inner-to-outer diameter of a myelinated axon) binned across the indicated axonal diameters. Results are from 12 animals (n = 6 WT, n = 6 Nestin-Het). Box plots show the 25th–75th quantiles (box), mean (red line), and whiskers delineate 3 standard deviations from the mean. e Immunostaining for myelin basic protein (MBP) reveals decreased myelination in callosal regions (dotted lines) of Anks1b Nestin-Het mice. Scale bar = 100 μm. (p = 0.00523). f Immunostaining for Olig2 reveals fewer oligodendrocytes per unit area. Scale bar = 100 μm. (p = 0.00299). For e, f: n = 8 Anks1b Nestin-Het, n = 8 WT, 4 sections from each mouse. a, b, e, f Error bars reflect mean ± s.e.m. a–f T-tests, two-sided; *, **, ***p < 0.05, p < 0.005, p < 0.0005. Source data for a, b, d, e, and f are provided as a Source Data file.
