Fig. 1: Assessment of cellular and organismal Polq^−/− phenotypes.

A Cartoon showing (left), Brca1 alleles and protein products with domain functions indicated; (Right), Brca1 functions in HR (simplified), the Brca1-Δ11 protein is defective for DNA end resection; the Brca1-CC protein is defective for Palb2-Brca2-Rad51 loading. B Western blotting for the indicated proteins in MEF cell lines. C Cartoon showing (left), process for generating CRISPR/Cas9 manipulated MEFs. (Right), Polθ protein domains and predicted effect of stop codon from sgRNA targeted exon 4 of Polq. D Polq gene mutations were binned according to whether frameshift mutations in both alleles (−/−) (green), or retaining at least one allele with a missense or one frameshift mutation (+/+, +/−) (blue). See Supplementary Data 1. E Mice with the indicated genotypes were intercrossed, and expected and observed genotypes were shown. A representative photograph of mice with the indicated genotypes is shown. See Supplementary Fig. 1e. P values are obtained from two-sided chi-square goodness of fit tests for the binomial of each genotype. F Mice with the indicated genotypes were intercrossed, and expected and observed genotypes were shown. P values were obtained from chi-square goodness of fit tests comparing expected and observed genotypes. Source data are provided as a Source Data file.