Fig. 2: Cell growth and chromosome analyses of Polq−/− genotypes.
From: Genetic separation of Brca1 functions reveal mutation-dependent Polθ vulnerabilities
A Growth curves were obtained for Polq−/− clones and control cells (sgGFP) for Brca1+/+, Brca1CC/CC, and Brca1Δ11/Δ11 MEFs based on confluence measurements obtained using a Sartorius Incucyte imager. Confluence and S.E.M. of three independent experiments are shown. ***p < 0.001; **p < 0.01; *p < 0.05; ns, not significant (unpaired, two-tailed t tests comparing Polq+/+ and Polq−/− confluence on each day). B Representative karyograms are shown for the indicated genotypes and marker chromosome counts are shown normalized to 40 chromosomes per metaphase. Each data point is an individual metaphase, n = 6, 4, 7, 9, 5, 4 (unpaired, two-tailed t tests). See Supplementary Table 1. C Example G-banded partial metaphase spreads from Brca1CC/CC, Polq−/− cells highlighting marker (mar) chromosome, fragments (frag), dicentric (dic), double minutes (dmin), and chromatid breaks (arrows) are shown from 1 experiment. D Brca1CC/CC, Polq+/+ and Brca1CC/CC, Polq−/− metaphase spreads were stained using mFISH and karyograms generated. The number of translocations per metaphase were quantified, n = 4, 6. Representative mFISH karyograms are shown, and a magnified image of a derivative chromosome consisting of segments from four different chromosomes is highlighted, far right. * indicate clonal marker chromosomes. See Supplementary Data 2, 3 and Supplementary Fig. 2d. Source data are provided as a Source Data file.
