Fig. 2: Mid-old specific gene expression in old-aged tissues.
From: Mid-old cells are a potential target for anti-aging interventions in the elderly
a–d Representative images of p21Waf1, SAA1, IL1β and SLIT2 IHC analysis in young and old-aged human colon and lung tissues are shown. “1” and “2” indicate high-magnification views of the original figure. Image “1” primarily depicts the epithelium and smooth muscle region, while image “2” depicts a stromal-rich region. Black arrows indicate stained cells in the stromal region. Yellow arrow in (d) indicate SLIT2-positive cells in epithelial region. Corresponding quantification dot plots are shown in the right panel of each figure. IHC results were presented as the percentage of positive cells in the stromal or epithelial region. The p value was calculated using the one-tailed Mann–Whitney U-test. The graphs in (b) (right tables) and (d) (lower right table) were represented as mean + SD. The graphs in (a), (b) (left tables), (c), and (d) (left tables) were represented as mean ± SD.
