Fig. 3: Single nucleus RNA sequencing (snRNA-seq) analysis of PFC in ZIKV offspring mice. | Nature Communications

Fig. 3: Single nucleus RNA sequencing (snRNA-seq) analysis of PFC in ZIKV offspring mice.

From: Brain methylome remodeling selectively regulates neuronal activity genes linking to emotional behaviors in mice exposed to maternal immune activation

Fig. 3

a The experimental scheme for snRNA-seq. b Expression of markers for each cell subtype in the mouse PFC. c 15 clusters across 16,135 cells from all cell types in cortex on a UMAP visualization. d Proportion of each cell cluster in ZIKV group vs. mock control. e Burden analysis of numbers of differentiated expressed genes (DEGs) in individual cell clusters. Box–whisker plot (displaying the Max/Min at the whiskers, the 75/25 percentiles at the boxes, and the median in the center line). N = 10 biological replicates per cell type. P values were calculated by two-tailed unpaired t-test. f GO analysis of DEGs in PFC of ZIKV offspring vs. mock control. DEGs refer to genes with consistent fold changes between snRNA-seq and bulk RNA-seq. The P value of the overrepresentation of a gene set was calculated using a Fisher’s exact test (FET) in each of these gene lists. g Violin plots showing differently expressed IEGs, including Egr1, 3, 4 and Arc in each cell subtype of the mouse PFC. Note the enriched IEG upregulation in excitatory neurons. Source data are provided as a Source Data file.

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