Fig. 5: HDAC8 activation induces changes in chromatin accessibility of Jun and MITF targeted genes.

A ChIP-Seq analysis was performed on HDAC8 expressing and EV expressing melanoma cells at both (a) promoter and (b) enhancer regions. c Cell lines were probed for acetyl-Histone3 and total-Histone 3 levels by immunoblot. Levels of acetyl-Histone3 were normalized to total-Histone3 and quantified by ImageJ. d A HOMER analysis was performed on HDAC8 and EV expressing cells. Shown are enriched transcription factor binding motifs in HDAC8 and EV expressing cells. e–g ATAC-Seq was performed on HDAC8 expressing and EV expressing cells. e A global analysis of all open chromatin was performed. A global analysis was performed on HDAC8 expressing and EV expressing melanoma cells at both (f) promoter and (g) non-promoter regions. h HDAC8 expression increases accessibility in Jun targeted genes and decreases accessibility of MITF targeted genes. A HOMER analysis was performed on ATAC-Seq data. HDAC8 was associated with increased accessibility at Jun binding sites while the EV control had increased accessibility at MITF binding sites. Source data are provided as a Source Data file.