Fig. 6: Spatial heterogeneity of extracellular matrix proteins across the AT/RT tumour at 833 µm spatial resolution.

a Cluster-map of AT/RT tissue generated by affinity network fusion (ANF) of core matrisome proteins as defined in MatrisomeDB. This clustering is spatially-aware, meaning the relative spatial locations of samples and protein abundance values are used to generate these clusters. The grey voxel represents one sample where no core matrisome proteins were detected. b Enriched MSigDB Hallmark gene sets within marker proteins (two-sided Wilcox test, Benjamini-Hochberg multiple testing correction threshold of 1%) of clusters shown in the cluster map. Significantly enriched hallmarks (one-sided hypergeometric test, Benjamini-Hochberg multiple testing correction threshold of 5%) for each cluster are indicated by the presence of circles. The size and colour of the circles represent the number of proteins contributing to that term and the adjusted p value of the enrichment, respectively. c Proteomic maps of selected extracellular matrix proteins and integrin receptors at 833 µm resolution. Normalised protein intensities are scaled separately for each protein. Grey = not detected.