Fig. 3: Elevating 3HAA protects C. elegans against oxidative stress and activates the Nrf2/SKN-1 oxidative stress response. | Nature Communications

Fig. 3: Elevating 3HAA protects C. elegans against oxidative stress and activates the Nrf2/SKN-1 oxidative stress response.

From: On the benefits of the tryptophan metabolite 3-hydroxyanthranilic acid in Caenorhabditis elegans and mouse aging

Fig. 3

a Knockdown or deletion of haao-1 or 1 mM 3HAA increases survival of C. elegans challenged with 20 mM paraquat. b 500 μM H2O2 reduces visible red color of 1 mM 3HAA in water. c 3HAA reduces H2O2 in water in a dose-dependent manner. d Deletion of haao-1 or 1 mM 3HAA represses the age-dependent increase in H2O2 secretion in C. elegans. e C. elegans lacking haao-1 have elevated endogenous H2O2 (HyPer). haao-1(RNAi) or 1 mM 3HAA increases fluorescence in C. elegans strains transgenically expressing f the skn-1::GFP fusion protein or g the gcs-1p::GFP or gst-4p::GFP promoter activity reporters. Hypomorphic skn-1 loss-of-function largely prevents lifespan extension from h haao-1(RNAi) or (i) 1 mM 3HAA. Colored text indicates change in mean lifespan relative to control. Error bars indicate standard error of mean. *p < 0.05, **p < 0.01, ***p < 0.001 (log rank test, panels a, h, i; ANOVA; panels c, d; Welch’s t test, panels f, g). Summary statistics are provided in Supplementary Data 1 and source data are provided in Source Data.

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