Fig. 2: chimeric-eCLIP-seq analysis identifies Ago2-associated, directly interacting pairs of miRNA and its target mRNA in the cisplatin-injured mouse kidney.

a Approach to determine directly interacting pairs of miRNA and its target mRNA (chimeric-eCLIP-seq) in the cisplatin-injured kidney, as well as to identify mRNAs (total RNA-seq) and miRNAs (small RNA-seq) expressed in matching kidney tissues. b Percentages of sequencing reads from total RNA-seq and small RNA-seq (blue) in the entirety of annotated mouse RNA. Percentages of sequencing reads from chimeric-eCLIP-seq reads are shown relative to the total kidney-expressed mRNAs and miRNAs (red). c The position of the miRNA reads mapped along the metagene of mRNA from chimeric-eCLIP-seq performed without UV crosslinking. 5’UTR, 5’ untranslated region. CDS, coding sequence. 3’UTR, 3’ untranslated region. d Display of miRNAs ranked by the abundance of miRNA-mRNA chimeric reads of the chimeric-eCLIP-seq. Measurements were made from biologically independent mice. n = 3 per group. Data are presented as Mean ± SEM. e Plots depicting overlaps between the chimeric-eCLIP-seq dataset of a given miRNA (miR-143-3p, miR-21a-5p, miR-30e-5p or miR-29b-3p) and miRDB target sets, which are ranked by fold enrichment. FDR, false discovery rate. Source data are provided as a Source Data file.