Fig. 1: A typical read coverage at 3’ termini in RNAtag-seq data.
From: TRS: a method for determining transcript termini from RNAtag-seq sequencing data
a Shown is the read coverage along the gene talB for three RNAtag-seq libraries (orange, red and cyan lines) mapped to E. coli K-12 MG1655 reference genome (NC_000913.3). The transcription start and termination sites (determined by SEnd-seq4) are marked by an arrow or a diamond arrow, respectively. The gene coding sequence is marked by a wide arrow containing the gene name. b Schematic representation of the RNAtag-seq protocol10. Briefly, the protocol involves the following steps: Random fragmentation of the RNA (black lines), RNA 3’ end adapter ligation, reverse transcription (gray lines), cDNA adapter ligation, PCR and sequencing. c For multiple transcripts of the same RNA (black lines), break positions created by the random fragmentation (blue slashes) result in randomly distributed 3’ termini except for the genuine 3’ terminus, which is always present. Consequently, the number of read starts (blue bars) at the genuine 3’ terminus is higher than at other 3’ termini, underlying the observed pattern of reads (black arrows).
