Fig. 6: Targeted Gas5 knockdown impairs fear extinction memory.
From: Fear extinction is regulated by the activity of long noncoding RNAs at the synapse
a Subcompartment-specific CIRTS-Gas5-mediated Gas5 knockdown leads to a reduction in endogenous Gas5 expression at the synapse (n = 7 independent biological replicates per group, two-tailed unpaired Student’s t test, t(7.681) = 3.158, p = 0.0142) *p < 0.05, with (b) no effect on Gas5 expression in the nucleus (n = 7 independent biological replicates per group, two-tailed unpaired Student’s t test, t(8.77) = 0.8795, p = 0.4026). c Schematic of the behavioral protocol used to test the effect of Gas5 knockdown in the ILPFC on fear extinction memory. CTX context, CS conditioned stimulus, US unconditioned stimulus. d There was no effect of Gas5 knockdown on within-session performance during fear extinction training (EXT Control, n = 13 independent biological replicates per group, EXT CIRTS-Gas5, n = 8 independent biological replicates per group, two-way repeated measures ANOVA, F (1,19) = 1.447, p = 0.2438). e There was no effect of Gas5 knockdown on the ability to express fear memory (RC Control vs RC CIRTS-Gas5); however, mice treated with CIRTS-Gas5 exhibit impaired fear extinction memory (RC Control, n = 10 independent biological replicates per group, RC CIRTS-Gas5, n = 11 independent biological replicates per group, EXT Control, n = 13 independent biological replicates per group, EXT CIRTS-Gas5, n = 8 independent biological replicates per group, two-way ANOVA, F3,38 = 8.995, p = 0.0001; Dunnett’s post hoc tests: RC Control versus EXT Control, CS1 ***p = 0.0007, CS2 ***p = 0.0004, CS3 ****p < 0.0001; RC Control versus EXT CIRTS-Gas5, CS1 p = 0.0994, CS2 p = 0.084, CS3 *p = 0.0158; RC Control versus RC CIRTS-Gas5, CS1 p = 0.4485, CS2 p = 0.7067, CS3 p = 0.9808). Error bars represent S.E.M.
