Fig. 5: MsrA is induced by methionine restriction via foxo.

a–c Time course (a) and the results (b, c) of the quantitative RT‒PCR analysis of MsrA in the gut (b) and abdomen (c). n = 4 for (b) and (c). For the statistics, a two-tailed Student’s t test was used. d Quantitative RT‒PCR analysis of MsrA in the guts fed with single amino acid-restricted diets for 24 h. n = 3. For the statistics, one-way ANOVA with Dunnett’s multiple comparison test was used. e Quantitative RT‒PCR analysis of MsrA in the guts fed a methionine-restricted diet for 24 h. Knockdown of foxo using the drug-inducible whole-body driver da^GS. RU486 (20 μM) was added to the medium. n = 3. For the statistics, one-way ANOVA with Holm–Šídák’s multiple comparison test was used. f Quantitative RT‒PCR analysis of MsrA in the gut. Overexpression of foxo for four days using an enterocyte driver NP1-Gal4 with temperature sensitive tub-Gal80^ts. The flies were fed a standard yeast-based diet. n = 4. For the statistics, a two-tailed Student’s t test was used. For all graphs, the mean and SEM are shown. Data points indicate biological replicates. Source data are provided as a Source Data file.