Fig. 3: The two-dimensional resolution distribution between retained and removed particles was compared.
From: A minority of final stacks yields superior amplitude in single-particle cryo-EM
The third iteration of CryoSieve achieved a retention ratio of 51.2% and a removal ratio of 48.8%, resulting in a similar number of particles for retention and removal. These two categories underwent CryoSPARC 2D clustering and averaging, i.e., 2D classification, with the number of 2D classes set to 50. All eight experimental datasets were tested. CryoSPARC reported the 2D resolution of each 2D class, along with the number of particle images belonging to it. We statistically analyzed the number of particles belonging to each 2D resolution and plotted histograms, demonstrating the difference between retained (steel blue) and removed (crimson) particles in terms of 2D resolution distribution. For TRPA1 and apoferritin, the bar with the highest resolution range was further finely divided and then plotted in a histogram, which is displayed to the right of the global histogram.
