Table 2 The finest subsets alleviate high-resolution amplitude decay, along with a comparison to their theoretical number of particle limit

From: A minority of final stacks yields superior amplitude in single-particle cryo-EM

Dataset

A

B

C

D

E

F

G

H

TRPA1

3.90

141.9

78.1 (63.8−)

198.5

147.3 (51.2−)

521

11,426 (21.9×)

43,585 (83.7×)

hemagglutinin

3.62

232.0

160.8 (71.2−)

226.9

146.4 (80.5−)

975

34,078 (35.6×)

130,000 (133.3×)

LAT1

3.11

132.6

96.0 (36.6−)

147.3

94.9 (52.4−)

6697

65,687 (9.8×)

250,712 (37.4×)

pfCRT

3.37

85.1

49.5 (35.6−)

235.8

66.8 (169.0−)

4212

4429 (1.01×)

16,905 (4.0×)

TSHR-Gs

2.96

92.9

61.7 (31.2−)

96.9

62.4 (34.5−)

9205

13,465 (1.46×)

41,054 (4.5×)

TRPM8

2.98

94.7

76.7 (18.0−)

110.1

82.2 (27.9−)

2200

13,789 (6.3×)

42,040 (19.1×)

apoferritin

1.81

70.5

58.0 (12.5−)

81.6

49.2 (32.4−)

74,530

80194 (1.08×)

382,391 (5.1×)

streptavidin

2.99

125.6

101.8 (23.8−)

90.4

69.3 (21.1−)

2152

7772 (3.6×)

23,991 (11.1×)

  1. A Half-maps resolution of the CryoSieve-retained particles (Å); B B-factor reported by CryoSPARC auto-postprocessing obtained from all particles in the final stacks (Å2); C B-factor reported by CryoSPARC auto-postprocessing obtained from the CryoSieve-retained particles with temperature decrease (compared with all particles) in brackets (Å2); D Rosenthal’s B-factor obtained from all particles in the final stacks (Å2); E Rosenthal’s B-factor from the CryoSieve-retained particles with temperature decrease (compared with all particles) in brackets (Å2); F theoretical number of particles limit at B = 50 Å2; G number of the CryoSieve-retained particles with folds of theoretical limit in brackets.; H number of particles in final stacks with folds of theoretical limit in brackets. Three datasets (pfCRT, TSHR-Gs and apoferritin) were emphasized by bold font as the number of particle images in the finest subset approaches the theoretical limit.