Fig. 6: Alternative reactions in Mb-catalyzed carbene transfer with BDK.
From: Mechanistic manifold in a hemoprotein-catalyzed cyclopropanation reaction with diazoketone
a Alternative products generated from the reaction of Mb(H64G,V68A) with BDK in the absence of olefin and in the presence of different alcohols as cosolvents. b GC trace of reaction in the presence of 3 mM Mb(H64G,V68A), 30 mM Na2S2O4, 100 mM BDK, in 50 mM potassium phosphate buffer (pH 7) with 10% MeOH, 16 h, room temperature, anaerobic conditions. See Supplementary Fig. 9 for additional data. * = unidentified product. c, d Time-course analysis of the formation of reduction byproduct 4 (c) and ethanol O-H carbene insertion product 7 (d) in the reaction with 3 mM Mb(H64G,V68A), 30 mM Na2S2O4, 100 mM BDK, in 50 mM potassium phosphate buffer (pH 7), 10% (v/v) ethanol.
