Fig. 3: Crosslinked VDAC1 dimers have phospholipid scramblase activity.
From: Phospholipids are imported into mitochondria by VDAC, a dimeric beta barrel scramblase
a VDAC1 in LDAO was cross-linked using EGS, or mocked treated with DMSO, and visualized by SDS-PAGE immunoblotting using antibodies against the N-terminal His tag. Representative dithionite (b) and BSA-back extraction (c) traces with cross-linked (VDAC1x) or mock treated (VDAC1) samples reconstituted into LUVs at a theoretical copy number of 30 proteins/vesicle. Normalized fluorescence traces are shown. These assays were performed 5 times with similar results. d Fraction of VDAC1-vesicles with scramblase activity (reconstituted at a theoretical protein/vesicle copy number of 30). Data points shown as individual points, as well as the mean ± 95% CI, n = 5, p < 0.0001 (two-tailed unpaired t test). After EGS cross-linking, nearly all vesicles that show channel activity also have scramblase activity.
