Fig. 3: SRRMs are located close to FAPs.

a The number of TIM4- and TIM4+ RMs detected by flow cytometry in skeletal muscle following CSF1R inh/wd (n = 7 mice for the control group, n = 3, 8, and 8 for the CSF1R inh/wd group on D0, D7, and D14, respectively; data were pooled from ≥3 experiments, ordinary one-way ANOVA). b Replacement rate of muscle RMs by blood progenitors following pharmacological depletion: The replacement rate of RMs was normalized to the percentage of chimerism in mononuclear myelomonocytic cells in the blood (n = 3 female parabiotic pairs per time point, data were pooled from ≥3 experiments, paired t test). c Immunofluorescent staining of Tibialis Anterior (TA) muscle sections and quantification of the distance between LYVE1+ SRRMs or LYVE1- RMs and PDGFRα-EGFP+ FAPs (n = 6 mice, pooled from 2 experiments; paired t-test). On average, we manually measured distances of approximately 575 CD68 + LYVE1- and 450 CD68 + LYVE1+ RMs from their closest FAPs in each mouse. d Immunofluorescent staining of TA sections and quantification of the distance between LYVE1+EdU+ or LYVE1+EdU- cells and PDGFRα-EGFP+ FAPs (n = 3 mice pooled from 2 experiments; paired t-test). On average, we manually measured distances of approximately 175 LYVE1+EdU- and 25 LYVE1+ EdU+ cells from their closest FAPs in each mouse. The figure displays two LYVE1+ EdU+ cells situated in proximity to FAPs. The cell on the left exhibits a dividing nucleus, as illustrated in greater detail in the inset.