Fig. 4: DPPIV+ FAPs form the niche of skeletal muscle SRRMs. | Nature Communications

Fig. 4: DPPIV+ FAPs form the niche of skeletal muscle SRRMs.

From: DPPIV+ fibro-adipogenic progenitors form the niche of adult skeletal muscle self-renewing resident macrophages

Fig. 4

a The number of LYVE1+ SRRMs and FAPs (PDGFRα-EGFP+) in TA sections (n = 4 mice pooled from two experiments, paired t-test). b Immunofluorescent staining of TA sections and quantification of the number of LYVE1+ SRRMs and DPPIV + PDGFRα-EGFP+ FAPs (top) and the percentage of DPPIV+ cells among all PDGFRα-EGFP+ FAPs or among PDGFRα-EGFP+ FAPs with a SRRM in their close vicinity (n = 5 mice pooled from 2 experiments, paired t-test, bottom). On average, approximately 800 FAPs per mouse were included for quantification. Among them, an average of about 100 FAPs had SRRMs in close vicinity. c Experimental design and gating strategy to sort DPPIV- and DPPIV+ FAPs for bulk RNA sequencing (top) and heatmap showing selected differentially upregulated genes in DPPIV+ FAPs compared to DPPIV- FAPs categorized by different signaling pathways (bottom).

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