Fig. 4: Interfacial Tse5-membrane binding delivers Tse5-CT toxin to the target membrane. | Nature Communications

Fig. 4: Interfacial Tse5-membrane binding delivers Tse5-CT toxin to the target membrane.

From: Structural and functional insights into the delivery of a bacterial Rhs pore-forming toxin to the membrane

Fig. 4: Interfacial Tse5-membrane binding delivers Tse5-CT toxin to the target membrane.

a (Upper panel) Schematic representation of molecular self-assembly of phospholipid molecules using the modified solvent-assisted lipid bilayer (SALB) method. (Lower panel) Selected QCM-D traces showing frequency (black) and energy dissipation (red) changes for 9:1 POPE/POPG bilayer formation. The consecutive steps are divided by dashed vertical lines: water flow, isopropanol exchange, DOPE/DOPG lipid-isopropanol addition and water exchange. b Representative QCM-D measurements of changes in frequency (upper graph) and dissipation (central graph) at the seventh overtone upon addition of 1 μM Tse5, Tse5-D1141A or Tse5-ΔCT in buffer TBS. QCM-D maximum change in frequency (left bottom graph) and dissipation (right bottom graph) measured for the seventh overtone after protein addition. Independent experiments were performed in triplicate (n = 3). The graphs show the mean values, the standard deviations (SD), and the value of each replicate. Statistical significance was evaluated with the ordinary one-way ANOVA with Tukey’s multiple comparison test. P-values of Tse5 vs Tse5-D1141A, Tse5 vs Tse5-ΔCT, and Tse5-D1141A vs Tse5-ΔCT are 0.0027, 0.0373, and 0.1011, respectively. P-value > 0.1234 (ns), 0.0332 (*), 0.0021 (**), 0.0002 (***), < 0.0001 (****). Plotting and data analysis were done with GraphPad Prism v9.5. c Plot showing changes in dissipation as a function of changes in frequency (ΔD/Δf) at the seventh overtone upon addition of 1 μM Tse5, Tse5-D1141A or Tse5- ΔCT in buffer TBS. The different ΔD/Δf slopes are indicated with lines for visual inspection.

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