Fig. 1: IL5+ ILC2s are required to maintain M2-like dermal TRMs which play a critical role in non-healing L. major infection.
a Representative flow cytometric analysis of ear isolates prepared from naive R5: ROSA26-LSL-tdTomato mice. CD45.2+CD11b-CD90.2+NK1.1--gated cells were analyzed for the expression of TCRβ and TCRγδ, and subdivided into DETCs (green; TCRγδhighTCRβ-), γδT cells (blue; TCRγδintTCRβ-), ILC2s (Red; TCRγδ-TCRβ-), and αβT cells (orange; TCRγδ-TCRβ+). Both CD2/CD3 and tdTomato expression were shown by overlaying color-coded populations in CD2-CD3 axes and histogram respectively. b tdTomato expression in ILC2s and (c) the absolute numbers of indicated cells recovered from ears of R5 (n = 8) vs. R5: ROSA26iDTR (n = 7) mice were measured at 12 days p.i. with 2 × 105 LmSd followed by DT treatment. d, e The same parameters are shown as above in ear isolates prepared from R5 (n = 4) vs. R5: Gata3f/f (n = 4) mice at day 12 p.i. with 2 × 105 LmSd, or (f) WT (n = 8) vs. RT: Gata3f/f (n = 8) vs. R5: Gata3f/f (n = 8) mice adoptively transferred with in 0.5 × 106 in vitro expanded, splenic ILC2s from WT mice, intravenously administered on day 0 p.i. with 2 × 105 LmSd. g Lesion development and pathology score over the course of infection with 103 LmSd metacyclic promastigotes in the ear dermis of Gata3f/f, Il4−/−, and R5: Gata3f/f animals (n = 8). h Parasite burdens were quantified at 12 weeks p.i. Values represent mean ± standard deviation (n = 8). *P < 0.05, **P < 0.01, and ***P < 0.01 by two-sided non-parametric Mann-Whitney test (b–e) and one-way ANOVA with Dunn’s posttest compared with Gata3f/f (f, g). Data are representative of two independent experiments (a–g). Source data are provided as a Source Data file.
