Fig. 5: TSLP promotes ILC2 infiltration and interaction with dermal TRMs during L. major infection. | Nature Communications

Fig. 5: TSLP promotes ILC2 infiltration and interaction with dermal TRMs during L. major infection.

From: Dermis resident macrophages orchestrate localized ILC2 eosinophil circuitries to promote non-healing cutaneous leishmaniasis

Fig. 5

a Representative flow cytometric analysis of isolates from epidermal and dermal layers of a naive ear skin to determine the tissue distribution of DETC, γδT, ILC2, and αβT cells. b Image obtained from an IVM of the ear of R5: ROSA26-LSL-tdTomato reporter mouse intravenously injected with efluor450 anti-CD31 Abs to label blood vessels, and its 3-dimensional surface rendering (right panel). Hair follicles are demarcated by dotted lines. The closest distance between dermal ILC2 and blood vessel was measured and plotted in the right panel. c Image obtained from an IVM of the ear of R5: ROSA26-LSL-tdTomato reporter mouse infected intradermally with 1 × 103 LmSd-GFP parasites and treated with either TSLP-neutralizing Abs or control IgG for 9 days. R5: ROSA26-LSL-tdTomato mice were injected with Cy5-ManoceptTM to label MRhigh dermal TRMs and efluor450 anti-CD31 Abs for blood vessels. Yellow-colored “R5 track” shows the paths followed by tdTomato+ ILC2s. Both the number and % surface of contacts per timepoint between ILC2s and dermal TRMs were analyzed and plotted in right panels. ****P < 0.0001 by two-sided nonparametric Mann-Whitney test (c). Data are representative of more than four independent mouse experiments (a–c). Source data are provided as a Source Data file.

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