Fig. 6: Ccl24-cre transgenic mice target MHCII^-MR^high dermal TRMs and TRMs in multiple tissues.

a Representative flow cytometric analysis of ear isolates from ROSA26-LSL-tdTomato mice vs. Ccl24-cre: ROSA26-LSL-tdTomato mice infected with LmSd-GFP for 8 days. b Live CD45.2⁺ singlets were gated into eosinophils, PMNs, inflammatory monocytes, moDCs, and dermal TRMs. The tdTomato⁺ cells in red were overlayed onto gatings. c Representative confocal image of ear dermis of infected Ccl24-cre: ROSA26-LSL-tdTomato mice which were intravenously injected with efluor450 anti-CD31 Abs and Cy5-Manocept^TM. d Quantification of percent tdTomato positive of dermal TRMs in (b) (n = 6). e Flow cytometric analysis of tdTomato⁺ cells in Ccl24-cre: ROSA26-LSL-tdTomato mice which were overlayed onto gatings for indicated organs. pgWAT; perigonadal white adipose tissue. The complete gating strategies are shown in Supplementary Fig. 6. Percent tdTomato positive of TRMs are labeled in red. f–j Immunohistochemistry analysis of Alexa488-Laminin Abs stained tissue sections or whole mount from Ccl24-cre: ROSA26-LSL-tdTomato mice which were intravenously injected with efluor450 anti-CD31 Abs and Cy5-Manocept^TM. G glomerulus. Tubules; Renal tubules which were visualized with filtered Cy5-Manocept^TM. Data are representative of more than three independent mouse experiments (a–c). See also Supplementary Fig. 5 and 6. Source data are provided as a Source Data file.