Fig. 5: Characterizing broadly neutralizing antibodies against HIV-1.
From: Interrogating ligand-receptor interactions using highly sensitive cellular biosensors
a Scheme depicting the BaL HIV-1 gp160 construct b Binding of CD4-Fc fusion protein to K562 cells (left panel) and K562 gp160 cells (right panel). c Scheme depicting interaction of gp160 complex on stimulator cell with CD4-ζ chimeric receptor on JE6.1 Nur77-mKO2 biosensor cells. d Expression of CD4 on JE6.1 Nur77-mKO2 and JE6.1 Nur77-mKO2 CD4-ζ. e, f JE6.1 Nur77 reporter cells and JE6.1 Nur77 CD4-ζ biosensor cells were co-cultured with stimulator cells as indicated (n = 4 experiments, performed in triplicates). e Representative histograms showing reporter gene expression of JE6.1 Nur77-mKO2 CD4-ζ biosensor cells cultured with indicated stimulator cells (data presented as Nur77-mKO2 FI) f Representative experiment, performed in triplicates. Data is presented as individual replicates with mean ± SD of gMFI Nur77-mKO2. Data of three repeat experiments is provided within Source Data file. g JE6.1 Nur77 CD4-ζ biosensor cells were co-cultured with K562 gp160 stimulator cells. The stimulators were preincubated with neutralizing antibodies as indicated (n = 2 experiments, performed in triplicates). Data of representative experiment is presented as replicate values with mean ± SD of gMFI Nur77-mKO2. Data of one repeat experiment and source data for this figure are provided in the Source Data file.
