Fig. 4: Hepatic overexpression of Slc30a10 reduces Mn content and Mn-dependent enzyme activity.

a–g Two-month-old C57BL/6 J male mice were i.v. injected with AAV expressing Flag-Slc30a10 (AAV-Slc30a10) or AAV-Control at low and high dose (2×10¹⁰ vg, and 2×10¹¹ vg per mouse, respectively), and fed NCD for 4 weeks before sacrificed (n = 5 ~ 7). Slc30a10 mRNA levels in the liver (a) (n = 6), Western blot analysis of the liver lysate with FLAG-Slc30a10 detected by anti-FLAG antibody, and protein expression of ARG1, Cu/Zn-SOD, and Mn-SOD in the liver whole lysate analyzed by Western blot with β-actin as a loading control (b), FLAG-Slc30a10 expression in the hepatocytes revealed by double immunohistochemical staining with anti-FLAG and anti-MDR1 antibodies with the indicated scale bars (c), liver Mn content (d) (n = 5 ~ 6), the activity of liver arginase (e) (n = 6), and the activity of Mn-SOD (f) (n = 6 ~ 7) and total SOD (g) (n = 6 ~ 7) in the liver. h The figure was created with Biorender.com. C57BL/6 J male mice on NCD were i.v. injected with AAV (2×10¹⁰ vg per mouse) and pretreated with i.p. CCl₄ or PBS as control prior to NH₄Cl challenge at the indicated time points (h). Plasma ammonia levels at the indicated time points after NH₄Cl injection with area under curve (i) (n = 6). Data represent means ± SEM. Two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.