Table 1 Names and genotypes of strains used in this study

WT

CBS7435

DasKO

CBS7435 das1Δdas2Δ

This study

GcvKO

CBS7435 das1Δdas2Δ gcv1Δgcv2Δ

This study

Shm1KO

CBS7435 das1Δdas2Δ shm1Δ

This study

ShmKO

CBS7435 das1Δdas2Δ shm1Δshm2Δ

This study

MisKO

CBS7435 das1Δdas2Δ mis1-1Δmis1-2&3Δ::loxP-kanMX-loxP

This study

GcvOE

CBS7435 das1Δdas2Δ P_FDH1GCV1 P_DAS2GCV2 P_AOX1LPD1 P_DAS1GCV3

This study

OtRedGlyOE

CBS7435 das1Δdas2Δ P_FDH1GCV1 P_DAS2GCV2 P_AOX1LPD1 P_DAS1GCV3 P_DAS1MIS1-1 P_AOX1SHM1 P_DAS2CHA1 P_DAS1ADE3 P_AOX1SHM2

This study

Shm1KO MeMisOE

CBS7435 das1Δdas2Δ shm1Δ P_DAS1Fhs P_CS1FchA P_TEF1MtdA

This study

Mut^-

CBS2612 aox1Δaox2Δ

Zavec et al. ¹⁷

Single MisKO

CBS7435 Δdas1Δdas2 Δmis1-1

This study

mitoOtRedGlyOE

CBS7435 Δdas1Δdas2 P_FDH1GCV1 P_DAS2GCV2 P_AOX1LPD1 P_DAS1GCV3 P_DAS1MIS1-1 P_AOX1SHM1 P_DAS2CHA1

This study

Das&Aox1KO

CBS7435 das1Δdas2Δ aox1Δ

Gassler et al. ¹⁵