Fig. 1: In vitro anti-inflammation and anti-senescence behaviors of sustainable H2 treatment of various senescent cells from aging bone.
From: Local H2 release remodels senescence microenvironment for improved repair of injured bone
Schematic illustration of testing the effects of sustained H2 treatment on BMSCs, macrophages and osteocytes collected from aging bone (a), the measurement of representative senescence biomarkers (ROS, HO-1, p16, p21, Ki67, γ-H2A.X, TAFs, SA-β-gal, IL-6, and IL-1β) in BMSCs, macrophages and osteocytes continuously incubated with or without H2 for 7 days (n = 6 biologically independent samples for ELISA measurement) (b), illustration of cellular senescence features and the anti-senescence, anti-inflammation, and regeneration-preserving effects of H2 for SME modulation (c). Data are means ± SD. P values in figure b were calculated by the two-tailed unpaired Student’s t test method (BMSCs: ****p < 0.0001 in day 3 for IL-6, ***p = 0.0005 in day 7 for IL-6, *p = 0.0181 in day 3 for IL-1β, *p = 0.0193 in day 7 for IL-1β; macrophages: ***p = 0.0008 in day 3 for IL-6, **p = 0.0065 in day 7 for IL-6, ns in day 3 for IL-1β, **p = 0.0012 in day 7 for IL-1β; osteocytes: ****p < 0.0001 in day 3 for IL-6, ***p = 0.0001 in day 7 for IL-6, *p = 0.0454 in day 3 for IL-1β, ns in day 7 for IL-1β; ns means no significant difference (p > 0.05)). a is created with BioRender.com. Source data are provided as a Source Data file.
