Fig. 4: Catalytic activities of lysine- and N-terminus-substituted PSMα3 variant fibrils.
From: Staphylococcus aureus functional amyloids catalyze degradation of β-lactam antibiotics
a Sequences of the PSMα3 variants. The modified residues in the variants are marked purple. Cryo-TEM images of the PSMα3 variant assemblies (concentrations 300 µM; 2-h incubation in DIW and addition of Hepes buffer). Bars correspond to 100 nm. The experiment was repeated two independent peptide samples that were prepared separately. b Nitrocefin degradation kinetics in the presence of PSMα3 variant assemblies (peptide concentrations 170 µM, initial nitrocefin concentration 107 µM). c Initial degradation rates, V0, at initial nitrocefin concentrations of 107 µM. Data presented in box-and-whisker plot, with mean line and quartile calculation using inclusive median, NPSMα3 = 14, Nα3-K6A = 3, Nα3-K9A = 4, Nα3K12A = 4, Nα3K17A = 4, Nα3-N.Ac = 3. The same color-coding was used in (b) and (c). Source data is provided as a Source Data file.
