Fig. 7: Effects of PSMα3 amyloid fibrils upon nitrocefin exposure in physiological scenarios.
From: Staphylococcus aureus functional amyloids catalyze degradation of β-lactam antibiotics
a Growth curve of S. aureus (black), S. aureus in the presence of nitrocefin (concentration of 100 µg/mL; red), co-addition of preformed PSMα3 fibrils (green), and in the presence of both PSMα3 fibrils and nitrocefin (blue). PSMα3 was preincubated in DIW and added to the growth media at a final concentration of 100 µM prior to addition of nitrocefin. Results are presented as an average ± SEM, N = 3 of independent bacteria samples that were prepared with/without independent PSMα, treated and analyzed separately. b Absorbance of degraded nitrocefin at 480 nm following incubation of nitrocefin (100 µg/mL) with washed biofilms produced by S. aureus, Salmonella enterica, or Escherichia coli (300 min incubation times). Results are presented in box-and-whisker plot, with mean line and quartile calculation using inclusive median, N = 4 of independent samples of biofilms that were grown independently and treated in parallel. Source data are provided as a Source Data file.
