Fig. 2: Single-molecule analysis of HRP-catalyzed chemiluminescent reactions.
a Images at increasing exposure times. Scale bar: 400 nm. b Statistics of photon counts per pixel for different exposure times. c Poisson and super-Poisson distribution of photon counts. Lambda is defined here as the variance and expectation of the distribution. The lambda value is 0.09, 0.95, 1.71, 4.12, 9.84 under the exposure time of 0.005 s, 0.05 s, 0.1 s, 0.2 s, 0.5 s, respectively. d Autocorrelation function Cτ of τoff from a single-molecule turnover trajectory. The x axis is the turnover index. The yellow solid line is the fit with a single exponential for decay constant moff of 0.214 detected turnovers. e TOF as a function of luminol concentration and the Michaelis-Menten equation fitting under three H2O2 concentrations. f Single-molecule Lineweaver-Burk plot corresponding to (e). The three lines intersect at a point to the left of the dashed line (abscissa <0). The chemiluminescence signal for different exposure times is acquired under 2.5 mM luminol, 15 mM H2O2, 0.23 mM 4-iodophenol, 5 nM HRP with EM Gain of 500. The chemiluminescence signal for different concentrations is acquired under 0.23 mM 4-iodophenol, 2.5 nM HRP with an exposure time of 5 ms and an EM Gain of 500. Source data are provided as a Source Data file.
