Fig. 5: Mass cytometry from the CARTELL cohort confirmed a shared protein profiles in CAR⁻ and CAR⁺ CD8⁺ cells and increase of NK-like T cell subsets following infusion.

a Dimensionality reduction and clustering using UMAP of both CAR⁺ and CAR⁻ CD8⁺ T cells identifying 9 clusters. Data are from the 8 patients in the CARTELL cohort. b Distribution of CARTELL samples in each identified cluster. c Selected protein expression plotted over UMAP. Darker colours represent greater expression. d Dot plot of scaled protein expression values for both CAR⁺ and CAR⁻ T cells in each cluster identified from the CARTELL samples. The size of each point represents the percentage of cells with non-zero expression. The colour represents the avg. (average) scaled log expression. e Differential protein expression showing differences between CAR⁺ and CAR⁻ T cells (N = 8). Shows are markers with adjusted p-value < 0.05, using edgeR (two-sided). f Selected protein expression values over time of CAR⁺ and CAR⁻ CD8⁺ T cells ⁽N = 8). Cells from the infusion product are removed. Adjusted p-values (paired two-sided Wilcox test with Bonferroni correction between time points) are shown and abbreviated to significance stars (****p < 0.0001) for readability. g Proportion of CD8⁺ subsets defined by CD57/T-bet expression in samples grouped by time point and split by CAR⁺ and CAR⁻ using N = 8 independent patients. All significant (p < 0.05) adjusted p-values (paired Wilcox test with Bonferroni correction between time points) are reported. Box plot whiskers extends to the minimum and maximum values, with the box encompassing the interquartile range (IQR), and the centre line indicating the median.