Fig. 5: ATR-CHK1 and CaMKK2 phosphorylate STN1 independently. | Nature Communications

Fig. 5: ATR-CHK1 and CaMKK2 phosphorylate STN1 independently.

From: CaMKK2 and CHK1 phosphorylate human STN1 in response to replication stress to protect stalled forks from aberrant resection

Fig. 5: ATR-CHK1 and CaMKK2 phosphorylate STN1 independently.

A Western blot of pS96 in HeLa and U2OS cells after co-inhibition of CHK1i (prexasertib, 1 μM, 1 h), and CaMKK2i (STO-609, 25 μM, 30 min), followed by 4 mM HU for 3 h. Representative blots from two independent experiments are shown. B SIRF detection of STN1 after co-inhibition of CHK1i (prexasertib, 1 μM, 1 h), CaMKK2i (STO-609, 25 μM, 30 min), and ATRi (VE821, 20 μM, 3 h). Cells were pulse labeled with EdU for 8 min, followed by 4 mM HU for 3 h. Scale bars: 10 μm. Representative images from two independent experiments are shown. P: One-way ANOVA. Red line: mean. n = ~100 cells were analyzed per sample in each experiment. Source data are provided in the Source Data file.

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