Table 1 Top 50 most highly induced bacterial genes in roots vs. matrix

From: Genome-resolved metatranscriptomics reveals conserved root colonization determinants in a synthetic microbiota

Strain ID|Gene ID

Gene name

Base meana

log2FCb

Adjusted Pc

Functiond

Root1280 | 1676

 

645

16.8

1.31e-16

Type-1 fimbrial protein, A

Root154 | 1230

 

857

16.4

6e-13

Unknown

Root1277 | 481

glmS

1157

16.3

4.21e-06

Glutamine-fructose-6-phosphate amidotransferase

Root154 | 382

 

838

16.1

6.35e-30

TonB-dependent receptor

Root70 | 3816

llpE

634

15.2

6.54e-25

Alpha-beta hydrolase fold-3 domain protein

Root154 | 1686

rhlE2

326

14.6

8.7e-08

DEAD DEAH box helicase

Root154 | 2017

 

1424

14.3

6.9e-10

Mycolic acid cyclopropane synthetase

Root154 | 1225

 

1096

14.1

2.78e-12

Pili and flagellar-assembly chaperone

Root70 | 4176

purU

345

14.1

3.01e-22

Formyltetrahydrofolate deformylase

Root154 | 768

 

1251

14.1

3.56e-08

Unknown

Root1277 | 4059

Echdc

257

13.9

5.93e-05

Enoyl-CoA hydratase/isomerase

Root154 | 1227

 

3781

13.8

4.16e-16

Unknown

Root123D2 | 2583

rskA

348

13.8

6.31e-06

Anti-sigma-K factor

Root1277 | 897

 

242

13.8

7.56e-05

Predicted periplasmic protein (DUF2271)

Root154 | 369

 

181

13.6

2.12e-06

Xylose isomerase-like TIM barrel

Root70 | 1346

 

215

13.4

1.77e-05

Nucleotidyltransferase

Root569 | 2962

pstC

467

13.4

3.17e-10

Polypeptide phosphate ABC transporter subunit

Root154 | 381

 

2868

13.4

3.25e-15

Lactoylglutathione lyase and related lyases

Root70 | 3634

 

202

13.2

2.65e-05

Drug metabolite transporter superfamily

Root1277 | 4057

 

1229

13.2

7.44e-09

Amine oxidase flavin-containing A

Root154 | 985

filA

135

13.1

3.71e-06

Membrane-bound metallopeptidase

Root559 | 706

 

219

12.9

1.4e-06

Belongs to the UPF0312 family

Root61 | 6

 

166

12.8

1.09e-06

Unknown

Root685 | 2415

 

608

12.8

1.54e-07

Copper chaperone PCu(A)C

Root123D2 | 3109

 

192

12.6

0.0001

Acetyltransferase (GNAT) domain

Root154 | 380

 

91

12.6

3.3e-06

PFAM TonB-dependent Receptor

Root102 | 1333

 

1413

12.6

2.35e-05

Unknown

Root102 | 4013

gabD

841

12.5

4.26e-05

Aldehyde dehydrogenase family

Root70 | 441

petB

754

12.4

2.61e-07

Cytochrome b6

Root322 | 1078

ilvA

238

12.3

1.5e-06

Pyridoxal-phosphate dependent enzyme

Root123D2 | 241

 

674

12.3

3.66e-07

Membrane fusion protein (TC 8.A.1) family

Root123D2 | 1229

 

154

12.2

0.0002

Unknown

Root1277 | 2958

dnaA

403

12.1

3.58e-05

Chromosomal replication initiator protein

Root172 | 4049

 

559

12.1

9.61e-05

von Willebrand factor (vWF) type A domain

Root149 | 1190

 

204

11.9

0.0007

Uncharacterized protein (DUF2059)

Root154 | 4234

rpsP

53

11.6

2.24e-05

Ribosomal protein bS16 family

Root322 | 1193

 

155

11.5

1.93e-05

Major facilitator Superfamily

Root322 | 2730

 

201

11.5

0.0005

MFS/sugar transport protein

Root149 | 1665

rplQ

123

11.4

0.0002

Ribosomal protein L17

Root670 | 456

dctA

883

11.4

3.32e-07

Aerobic C4-dicarboxylate transporter

Root322 | 1563

 

136

11.4

1.93e-05

Unknown

Root149 | 609

atpF

115

11.3

0.0002

ATP synthase subunit b

Root569 | 2421

aspA

1685

11.3

1.13e-14

Aspartate ammonia-lyase

Root559 | 1423

rnpA

90

11.3

5.94e-05

Ribonuclease P protein component

Root559 | 650

 

99

11.2

0.0001

Unknown

Root322 | 2077

yigZ

136

11.2

0.0001

Uncharacterized protein family UPF0029

Root61 | 2410

hmuT

253

11.1

5.36e-06

Periplasmic binding protein

Root61 | 2017

 

62

11.0

0.0001

Domain of unknown function (DUF4407)

Root61 | 1037

 

878

11.0

3.45e-10

Domain of unknown function (DUF4442)

  1. aDESeq-corrected average number of reads.
  2. bLog2 fold change.
  3. cDESeq2 Wald test P value adjusted for false discovery rate.
  4. dMost precise annotation available for the gene.