Fig. 4: Mechanism of PIP2-induced βarr1 partial activation.
From: Distinct activation mechanisms of β-arrestin-1 revealed by 19F NMR spectroscopy
a 19F NMR spectra showing the effects of PIP2 binding at different labeling sites of the C-domain. b Summary of the effect of PIP2 binding on different labeling sites mapped onto the βarr1 structure. c Top view of the βarr1 structure showing the possible mechanism of PIP2-induced activation. The PIP2 binding residues are colored in pink. Residues perturbed or unperturbed by PIP2 binding are shown in red and blue, respectively. d A schematic illustration showing that PIP2 binding induces enhanced dynamics in the C-domain and unlocks the interdomain restraints at the back side of βarr1.
