Fig. 5: 19F CEST experiments reveal an intermediate state in various structural regions. | Nature Communications

Fig. 5: 19F CEST experiments reveal an intermediate state in various structural regions.

From: Distinct activation mechanisms of β-arrestin-1 revealed by 19F NMR spectroscopy

Fig. 5: 19F CEST experiments reveal an intermediate state in various structural regions.The alternative text for this image may have been generated using AI.

a–e 19F CEST profiles in the basal, PIP2-bound and V2Rpp-bound states of βarr1 obtained for the D390C (a), E404C (b), and T6C (c) sites in the CT and TE regions and for the E206C (d), L334C (e) sites in the C-domain. The corresponding 1D 19F spectra are shown for comparison. f 19F CEST profiles of the gate loop H295C site obtained in the basal, PIP2-bound, V2Rpp-bound and ΔCT states. The corresponding 1D 19F spectra as well as that of the 1–395 construct in its basal state are shown for comparison.

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