Fig. 5: Complementary PNAs change protein-binding property and intracellular fate of ssASO. | Nature Communications

Fig. 5: Complementary PNAs change protein-binding property and intracellular fate of ssASO.

From: Dynamic and static control of the off-target interactions of antisense oligonucleotides using toehold chemistry

Fig. 5

a Schematic illustration of competitive protein pull-down assay, where ASO binding proteins are indicated using colored balls, and biotin tags is indicated by the black circles at the ends of ssASO or BRO. b, c Identification of liver-derived proteins bound by CBB staining for mPCS1b ssASO and mPCS1b/PNA(C8) BRO (n = 3 independent experiments) (b) and for hApo1b and hApo1b/PNA(C5−13) BROs (n = 2 independent experiments) (c). d Classification of identified binding proteins using LC/MS proteomic analysis. e Intracellular localization of Alexa647-labeled mPCS1f or mPCS1f/PNA(C8) BRO visualized using confocal microscopy (n = 2 independent experiments). Source data are provided as a Source Data file.

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